Western Blot analysis of beta Actin in HeLa cells using a beta Actin Recombinant Monoclonal antibody (Product # MA5-42946) at a dilution of 1:10,000-1:320,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 180s.